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Hiload 16/600 superdex 200 pg manual |
HiLoad 16/ Superdex 75 pg - 1 x ml - :
Product Description Details. We Accept Secure ways to pay by credit card or with a PayPal account. Featured - [more]. Latest News [View All]. Have you seen Superdex prep grade. Fractionation range M r. Superdex 30 prep grade. Superdex 75 prep grade. Spherical composite of cross-linked agarose and dextran.
Average particle size. Chemical stability. Storage temperature. Prepacked HiLoad Columns. Bed volume. GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Each column is expertly packed and individually tested. Extended embed settings. You have already flagged this document. Thank you, for helping us keep this platform clean. The editors will have a look at it as soon as possible.
Self publishing. Share Embed Flag. TAGS superdex prep hiload column columns healthcare prepacked volume separation proteins www. You also want an ePaper? Superdex prep grade is a matrix of dextran and highly cross-linked agarose. All are prep acked to mm bed heights in 16 mm or 26 mm diameter columns.
This combination of prep acked convenience and reproducibility make HiLoad Superdex prep grade columns a confident choice for fast, high-resolution gel filtration at prep arative scale. Superdex gel filtration media are produced by the covalent binding of dextran to highly cross-linked porous agarose beads. HiLoad Superdex 30, 75, and prep grade columns bring convenience and high resolution to gel filtration.
Steep selectivity curves give unmatched resolution for biomolecules in the molecular weight range up to 10 for Superdex 30 prep grade Fig 1 , to 70 for Superdex 75 prep grade , and 10 to for Superdex prep grade Fig 2. Myoglobulin, 1. IgG, 0. Ferritin, 0. Selectivity curves from Superdex 30 pg, Superdex 75 pg, and Superdex pg.
Table 1 summarizes the characteristics of the media and columns. Comparison of the selectivity of Superdex 75 prep grade and Superdex prep grade for model proteins. Superdex 75 prep grade A gives excellent resolution of the three proteins in the M r range 17 to 67 while the two largest elute together in the void volume. Superdex prep grade B resolves these two largest proteins. The ferritin 5 contains aggregates and thus results in a double peak.
All strong oxidizing agents should, however, be avoided. Extensive studies, including long-term exposure to harsh chemical treatment, have demonstrated that Superdex prep grade has extremely high chemical stability. Figure 5 illustrates the long-term stability of Superdex prep grade to 0.
Nonspecific interaction Studies have demonstrated varying degrees of nonspecific interaction between the chromatography media and acidic, as well as basic proteins in the absence of salt. Such interactions are negligible in salt concentrations between 0. Low sample volumes give high resolution even at high flow rates. Superdex 30 prep grade , Superdex 75 prep grade , and Superdex prep grade may be used in aqueous solutions over the range pH 3 to 12 for continuous operation and over the range pH 1 to 14 for cleaning-in-place CIP.
Performance of Superdex prep grade measured as K av values of a protein mixture after repeated treatment with A 0. After each exposure period the K av value was determined for a test mixture of proteins.
Following an accumulated exposure time of h, the exposure periods were increased to 16 h. Even after more than h accumulated exposure, K av values did not change significantly. Each has a precision bore borosilicate glass tube and a fitted thermostatic jacket. Dead volumes make up less than 0. Volumetric Pipettes. Pipette Tips and Racks. All Pipets, Pipetters and Tips. Syringes and Needles. Syringe Filters. Sharps Containers. All Syringes and Needles. See All Categories. Please sign in to view account pricing and product availability.
Sign In Don't have a profile? Brand: Cytiva Add to basket. Description Description Superdex prep grade pg is produced by covalent bonding of dextran to highly cross-linked agarose. Dextran component determined the separation properties of these media.
Steep selectivity curves give gppd resolving power for peptides and proteins in the molecular weight range Mr 10 Superdex pg. PKeep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. PKeep cool. Special Offers. Sample Volume. Content And Storage.
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